HEALTH

Best practices for storing peptides

Are you interested in how to store peptides in order to get the most out of them? Proper peptide storage is critical for the accuracy of lab findings. Proper storage can keep peptides safe for years while also protecting them from contamination, oxidation, and degradation, all of which may invalidate your peptides and research based on them. The stability and integrity of peptides may be extended regardless of composition by recognizing and applying the best methods for peptide storage.

 

Peptides must be kept cool and far from light after they have been received. Short-term cooling at 4C (39F) is normally suitable if the peptides will be employed within the next few days, weeks, or months. Lyophilized peptides have a long shelf life of up to several months when stored at room temperature, making them ideal for short-term use.

 

However, peptides should be stored in a freezer at -80C for many months to years if possible (-112F). For long-term storage of peptides (months or years), freezing is the best option.

 

Repeated freezing and thawing should be avoided at all costs. By doing this, the peptide becomes more degradable. Defrosting cycles in frost-free freezers may cause significant temperature fluctuations; hence they should be avoided while storing peptides.

 

Protection Against the Effects of Oxidation and Moisture

It’s critical to keep peptides dry and clean at all times. When a peptide is used shortly after it has been removed from the freezer, moisture contamination is more likely to occur. Allow the peptide to get to room temperature before opening to avoid moisture absorption from the air on the cold surface or within the container.

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It’s also critical to keep peptides out of the air as much as possible. The container of a peptide must thus be kept closed at all times. In order to limit peptide oxidation, seal the container with an inert gas (such as nitrogen or argon) once the needed quantity of peptide has been withdrawn. Sequences C, M, and W (tryptophan) are particularly vulnerable to oxidation in the presence of air.

 

Given that repeated freezing and thawing of peptides, as well as exposure to air, may drastically degrade their long-term stability, many scientists prefer to calculate the quantity of peptide needed for each experiment before aliquoting it into separate vials. Preventing peptide degradation is a valuable skill to have.

 

Peptide Solution Storage

When possible, avoid keeping your peptides in solution to ensure their long-term stability. A solution’s shelf life is much smaller than a dry powder’s, and wet peptides are far more susceptible to bacterial destruction. There is a low shelf life for peptides that include Cys, Met, Trp, Asp, Gln, and N-terminal Glu.

 

The only exception to this is if you need to preserve peptides, then you must use clean buffers at pH 5-6, and you must split the peptide solution into aliquots to prevent freezing and thawing again. While stored in a refrigerator at 4C (39F), peptide solutions are typically durable for up to a week, but those peptides with intrinsic instability should be frozen when not in use to ensure long-term stability.

 

Containers for Storing Peptides

Peptide storage containers must be free of contaminants, transparent, and physically robust. They should also be chemically robust, and the right size for the peptide content. Glass vials and plastic vials are both prevalent, with plastic vials ranging from those made of polystyrene to those made of polypropylene, depending on their composition. When it comes to chemical resistance, polypropylene vials beat out polystyrene because the former is transparent and the latter is chemical resistant.

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High-quality glass vials provide all the necessary features for a peptide storage container, yet peptides are occasionally sent in plastic vials to prevent breakage during shipping. Even so, peptides may be moved from a plastic to a glass container, or vice versa, if required.

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